This game marked the debut of Birdo, and also Princess Daisy's re-entries into the Mario series. In this game, Birdo competed as a Speed player, and was one of the new girls. In the intro of Mario Tennis, she is seen entering the court after Paratroopa, and she faces off against Princess Peach in the tournament. Later, she is seen with Paratroopa and Yoshi, when Bowser interupts the fun.
Also she have her own court (Slow but bouncy) which can be unlocked when the player win the Star Cup with Birdo in doubles. Also, when the player chose Birdo in the double tournament mode, Daisy would be her default partner.